My lab focuses on deciphering the molecular events connecting glycogen metabolism to cellular metabolism, signaling, and physiology, with a strong emphasis on the progression of neurodegenerative diseases and Glycogen-rich class of cancer.
My scientific philosophy is that I want to find true, clinically relevant and widely applicable mechanisms of disease. Therefore, my lab employs multiple models, from cell-free to animal models, multiple techniques, including metabolomics, histology, genetic manipulations, and pharmacological approaches to tackle complex biological questions
De novo synthesized glycogen accumulates in the nucleus of non-small cell lung cancers
Nuclear glycogen provides a carbon pool for histone acetylation
Nuclear glycogenolysis is dependent on translocation of glycogen phosphorylase
Glycogen phosphorylase translocation is regulated by the E3 ubiquitin ligase malin
Our lab established a unique combination workflow of targeted metabolomics using gas-chromatography, liquid-chromatography, capillary-electrophoresis, MALDI, and DESI mass spectrometry coupled to inhouse high throughput software with rigorous quality control and normalization steps to study perturbations in metabolism.
MALDI Mass Spectrometry Imaging
Matrix-assisted laser desorption/ionization mass spectrometry (MALDI Imaging) has entered the field of tissue-based research by providing unique advantages for analyzing tissue specimens in unprecedented detail. A broad spectrum of analytes ranging from proteins, peptides, protein modification, small-molecule metabolites, drugs, and their metabolites, as well as pharmaceutical components, lipids, and other analytes, are accessible by this in situ technique.
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